Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 18)

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zero the UV or QuadTec UV/Vis trace by clicking on the Zero baseline button
in the appropriate box. This button may be selected at any time.
c. To scale the on-screen chromatogram trace display axes, use the scroll bars
located on the left and right axes of the chromatogram window.
d. To enlarge the view select the Resize button to the right of the
chromatogram display.
Start the Run
a. Ensure that sufficient tubes are in the fraction collector rack (approximately 14).
The drophead will automatically move to tube 1 when the run is started.
b. Ensure that the AVR7-3 valve is in the LOAD position (position L). If it is not,
return to the Manual Screen by clicking the toolbar Manual button and click
on valve position L.
c. Ensure that the 50 µl sample loop is connected to ports 3 and 6 of the inject
valve. Completely fill the loop with protein standard via port 2 using the
syringe and needle provided. Do not remove the syringe from the injection
port after filling the loop or the sample will siphon to waste.
d. To launch the Run, click on the green Start toolbar button. The sample will
be loaded automatically.
e. When the run is finished, the pumps automatically stop and a “Run Finished”
message appears in the bottom right of the status bar.
f. Figures 5 and 6 show typical run screens and chromatograms for this
separation using the UV or QuadTec UV/Vis detectors.
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