Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
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Table of Contents
Introduction .......................................................................................1
1.
Starter Kit Components ..............................................................1
2.
Materials You Will Need...............................................................1
I. BioLogic DuoFlow System ....................................................3
Section 1. DuoFlow System Preparation .........................................3
1.1
Prime the Workstation Pumps.....................................................5
1.2
Move the AVR7-3 Inject Valve to the Purge Position ....................5
1.3
Purge the Workstation Pumps ....................................................5
1.4
Manual Control of the Workstation Pumps ..................................6
1.5
Flush the System Through to the Fraction Collector ....................6
BioFrac Fraction Collector
1.6
Turn on the UV Lamp ..................................................................6
DuoFlow UV Detector
QuadTec UV/Vis Detector
1.7
Manual Screen Chromatogram Window......................................7
1.8 Status
Bar...................................................................................7
Section 2. Anion Exchange Separation of Protein Standards........8
2.1
Overview of the Procedure..........................................................8
2.2 Prepare
Buffers...........................................................................9
2.3 Prepare
Sample ..........................................................................9
2.4
Install UNO Q1 Column ............................................................10
2.5
Prime the Pumps and Equilibrate the UNO Q1 Column ............10
2.6
Create a New Method...............................................................10
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