Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 6)

3
I. BioLogic DuoFlow System
Section 1. DuoFlow System Preparation
When the DuoFlow system is turned on, the Manual screen is displayed (see
Figures 1 and 2). This screen displays instrument control panels that provide
direct control of the pumps, valves, fraction collector, UV detector, QuadTec
™
UV/Vis detector, and Econo ™ Gradient Pump. The arrow button in the upper
right corner of the detector control panel toggles between the UV and QuadTec
detector control panels. Only those instruments connected to the system will be
displayed.
BioLogic Duo-Flow - - - no method -
Edit
Method
New
Method
New
Run Browser Manual Setup Setup Protocol
wash load 1 2
Run Notes Report PostRun Log
Advance
Fraction Collector: BioFrac
START
Gradient Pump: F10
Inlet A
1.00
50
50 Inlet B
Flowrate ml/min
%
%
High
limit
0
700
Low
limit psi.
AVR7-3 at port 4
Workstation Valves
SVT5-4 at port 1 SVT3-2 at port 3 SV5-4 at port 2
P L 2 1
2 1
3 4
2 1
3 4
STOP START STOP START STOP
Set
Event Mark
OFF ON
psi.
Econo Gradient Pump 1
EGP %B
1.000
0
0.00 % Split
Flow
Direction
Flowrate ml/min
%
%
Set
Tube number:
ml
Volume left:
Mode: System Local
Conductivity range
(mS/cm):
Mode: System Local
Start Tube:
End Tube:
Fraction size:
1
1
20
1.00
5
1.0
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
AVR9-8 at port 6
3 7
8 2
6
5
4
I
UV Detector
Chart Recorder
ON OFF
Zero Baseline
100% Buffer B
0 2 4 6 8 10
Minutes AU mS/cm
1.50
1.00
0.50
50
0.00 0.0
400.0
300.0
200.0
100.0
Fractions UV Conductivity
Clear
Traces
Resize
UV range (AU):
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
0.00 ml/min 50 %B
Gradient Pump: F10 UV Conductivity
1 psi 0.2583 AU 0.000 mS/cm
QuadTec Econo Gradient
Pump
SIM1/pH SIM1/SIG
Settings
File View Utilities Options Window Help
Bio-Rad
Web
Rack: F1 (12-13 mm tubes)
Zero baseline for the UV detector
Fig. 1. Manual Control Screen with UV detector
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