Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 28)

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Step 4 Prime the workstation pumps and inlet valves, and equilibrate the
column
Step 5 Write a method
a. Program in the instrument Setup
b. Program the method Protocol
c. Load sample into 50 µl loop
d. Select Run
e. Select Start
4.2 Prepare Solutions
During solution preparation, wear appropriate laboratory protective clothing,
including eye protection and gloves. Avoid skin and eye contact with starter kit
solutions. In case solutions come in contact with eyes, rinse immediately with
plenty of water and get medical advice.
Note: Solutions A, and A
2
are diluted to 500 ml, solution B
2
is diluted to 125 ml.
Solution A1
a. Empty the contents of the bottle labeled Solution A1 into a 500 ml graduated
cylinder and add filtered, high-quality water to a 500 ml volume.
b. Place the contents of the graduated cylinder into a 1 L side-arm flask and
drop in a stirbar. Cap the side arm flask, place it on a stirplate and connect it
to a vacuum source. Degas the solution for approximately 15 minutes with
gentle stirring.
c. When degassing is complete, pour the buffer into a bottle and label as
"Solution A1 50 mM Tris-HCl".
Solution A2
a. Empty the contents of the bottle labeled Solution A2 into a 500 ml graduated
cylinder and add filtered, high-quality water to 500 ml. Caution: pH of
Solution A2 is approximately 10.5.
b. Place the contents of the graduated cylinder into a 1 L side-arm flask and
drop in a stirbar. Cap the side arm flask, place it on a stirplate and connect it
to a vacuum source. Degas the solution for approximately 15 minutes with
gentle stirring.
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