Fig. 6. run screen quadtec uv/vis traces – Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual

Page 20

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background image

17

Advance

Frac. Collector

Divert Valve

1

3

2

4

5

6

7

8

9

10

11

12

13

14

100.0% Buffer B

UV Detector

Set

UV Range

Zero

Baseline

Event
mark

Protocol: > 1 0.00 Collection Fractions of size 2.00 ml during entire run

00:00:00

00:02:00

00:04:00

00:06:00

Hr:Min:Sec

AU

mS/cm

Collect

Waste

Grad. Pump

Chart Recorder

700

High psi

0

Low psi

QuadTec

Zero

Baseline

1.0

0.0

40.0

50.0

30.0

20.0

10.0

-10.0

Fractions

Conductivity

QuadTec (280 nm)

Full View

0.100

0.075

0.050

-50.0

0.0

0.025

-0.000

BioLogic Duo-Flow - Demo Chromatography - Standard UV Detector - Sample Run

File

View

Utilities

Options

Edit

Method

New

Method

New

Run Browser

Manual Setup Protocol

wash

load

1

2

Run Notes

Report

PostRun Log

Window

Help

Settings

WL1 - 280nm

0.2223 AU

Flow Rate

EGP %B

0 %B

0.00 ml/min

0%

% Split

Run Time

0:00.0

Run Volume

0.0 ml

Step Time Left

Fraction Vol. Left

Valve Info

0.00ml/min

50 %B

Maximizer + Gradient Pump: F10

0.15 AU

1.15 AU

0.30 AU

Conductivity

0 psi

0.2580 AU

3.63 mS/cm

QuadTec

Econo Gradient

Pump

SIM1/pH

SIM1/SIG

0.000 Volt

8.05 pH

Edit

Bio-Rad

Web

WL2 - 260nm WL3 - 214nm WL4 - 405nm

Fig. 6. Run screen QuadTec UV/Vis traces

4006208B.qxd 6/28/2004 11:03 AM Page 22

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