Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 4)

1
Introduction
This instruction manual and starter kit contents may be used for the BioLogic
DuoFlow system and the BioLogic DuoFlow Maximizer
™ and Pathfinder ™
chromatography systems. The use of the starter kit with these systems is
described in Sections 1 and 3, respectively.
1. Starter Kit Components
This starter kit contains the following items for running a separation:
• 50 ml of buffer A, 250 mM Tris-HCI buffer, pH 8.1 (10x concentrate)
• 50 ml of buffer B, 250 mM Tris-HCI buffer, pH 8.1, plus 5.0 M NaCl (10x concentrate)
• 50 ml of Maximizer solution A1, 500 mM Tris-HCl (10x concentrate)
• 50 ml of Maximizer solution A2, 500 mM Tris base (10x concentrate)
• 50 ml of Maximizer solution B2, 5.0 M NaCl (2.5 x concentrate)
• One vial of anion exchange protein standard (catalog #125-0561)
• One 1 ml disposable sample injection syringe
• One 50 µl sample loop
The chromatographic separation for this kit requires approximately 6 minutes.
2. Materials You Will Need
In order to prepare the starter kit buffer solutions you will need the following
materials:
• Filtered high-quality water (i.e., HPLC grade water)
• One 500 ml graduated cylinder
• One 1 L side-arm flask
• Stirbar and stirplate
• Vacuum source for degassing
• Two 500 ml bottles
• Fraction collection tubes, 13 x 100 mm (at least 14 tubes)
• 100 ml beaker
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