Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 25)

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BioFrac Fraction Collector
The BioFrac fraction collector has two operating modes:
• System—Controlled by the BioLogic DuoFlow system
• Local—Controlled from its own faceplate in stand-alone mode
Ensure that the System button is selected.
When in System mode, the fraction collector control panel on the Manual screen
will show fields for Rack type, Start tube, End tube, Fraction size, Tube number,
Volume left, a toggle button for Start and Stop, and a button for Advance (see
Figures 7 and 8).
3.6 Turn on the UV Lamp
DuoFlow UV Detector
a. The UV lamp automatically turns on when you turn on power to the
workstation. The UV lamp can be turned on and off by clicking the On and
Off buttons from the UV detector control panel on the Manual screen (see
Figure 7). Check that the lamp is on; the mercury lamp requires
approximately 30 minutes to warm up.
b. Select the Zero Baseline button to zero the UV signal. The Status bar along
the bottom of the screen provides AU output for the detector. Ensure that it
goes to zero when you select the zero baseline option.
QuadTec UV/Vis Detector
a. The QuadTec detector should be powered On before starting the BioLogic
software. If the QuadTec detector is not powered up, exit the software, power
up the QuadTec detector, and restart the software. When connection is
completed, “SLAVE” appears in the upper left corner of the QuadTec faceplate.
b. From the QuadTec detector control panel on the Manual screen (Figure 7),
set the four wavelengths of the QuadTec detector to 280, 260, 214, and
405 nm. Select Set. The active wavelength will appear in the lower screen
status bar.
c Click the Zero Baseline button to zero the four UV/Vis signals. The Status bar
along the bottom of the screen provides AU output for the detector. Ensure
that it goes to zero when you select the zero baseline option.
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