Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
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3.3 Purge the Workstation Pumps
a.
Make sure that the inject valve is in the Purge position.
b.
Select solutions A2 and B2 from the Valve Port Select buttons on the
Maximizer faceplate.
c.
Press the Purge buttons A then B on the front of the workstation. The
workstation pumps will run at 10 ml/min and the indicator light will flash green.
d.
Run each pump for 2 minutes and then press the Valve Port Select buttons
on the Maximizer faceplate to select solutions A1 and B1. Run each pump
for 2 minutes more and then press the Purge buttons to stop the pumps.
3.4 Manual Control of the Pumps
The workstation pump parameters can be set from the Manual screen
Maximizer+Gradient Pump control panel either by clicking in the appropriate field
and entering a value from the keyboard or by using the arrows. The appearance
of the these control panels depends on whether the instrument is in Buffer
Blending or Non-Buffer Blending mode. Figures 7 and 8 show examples of the
respective Buffer Blending and Non-Buffer Blending control panels. From these
control panels you can set the flow rate, pressure limits, and buffer composition.
You can switch between these two panels by pressing in the toolbar and
checking or unchecking the “Use Buffer Blending” box on the Maximizer Buffer
Blending Setup screen.
To start the pump, click the Start button. Note that the running man icon will start
running. To change the pump parameters while the pump is running, enter the
new value and then click on the Set button. Pressure limits can be adjusted to
match the pressure limits of a column. If the pressure limit is exceeded, the pump
will stop and an alarm will sound. If you are using an UNO Q1 column, set the
high limit to 700 psi and the low limit to 20 psi.
3.5 Flush the System Through to the Fraction Collector
With the gradient pumps stopped, move the AVR7-3 inject valve back to position
L (Load) by clicking L (AVR7-3) on the Manual screen.
From the gradient pump control panel on the Manual screen, set the pump flow
rate at 1.0 ml/min and start the pump. Water will flow through the UV or QuadTec
and conductivity flow cells to the fraction collector, as described below.
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