Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
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1.4 Manual Control of the Workstation Pumps
The workstation pump parameters are set from the Manual screen either by
clicking in the appropriate field and entering a value from the keyboard or by
using the arrows. You can set the flow rate between 0.01 to 10 ml/min and the
gradient composition between 0 and 100% B.
To start the pump, click the Start button. Note that the running man icon will start
running. To change the pump parameters while the pump is running, enter the
new value and then click on the Set button.
Pressure limits can be adjusted to match the pressure limits of a column. If the
pressure limit is exceeded, the pump will stop and an alarm will sound. If you are
using an UNO
™
Q1 column, set the high limit to 700 psi and the low limit to 20 psi.
1.5 Flush the System Through to the Fraction Collector
With the gradient pumps stopped, move the AVR7-3 valve back to position L
(Load) by clicking L (AVR7-3) on the Manual screen.
From the gradient pump control panel on the Manual screen, set the pump flow
rate to 1.0 ml/min and start the pump. Water will flow through the UV or QuadTec
and conductivity flow cells to the fraction collector, as described below.
BioFrac
™
Fraction Collector
The BioFrac fraction collector has two operating modes:
• System—Controlled by the DuoFlow system
• Local—Controlled from its own faceplate in stand-alone mode
Ensure that the System button is selected.
When in System mode, the fraction collector control panel will show fields for
Rack type, Start tube, End tube, Fraction size, Tube number, Volume left, a
toggle button for Start and Stop, and a button for Advance (see Figures 1 and 2).
1.6 Turn on the UV lamp
DuoFlow UV detector
a.
The UV lamp automatically turns on when you turn on power to the
workstation. The UV lamp can be turned on and off by clicking the On and
Off buttons from the UV detector control panel on the Manual screen
(see Figure 1). Check that the lamp is on; the mercury lamp requires
approximately 30 minutes to warm up.
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