Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
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II. DuoFlow Maximizer and Pathfinder Systems.....................18
Section 3. System Preparation.......................................................18
3.1
Prime the Workstation Pumps ..................................................20
3.2
Move the AVR7-3 Inject Valve to the Purge Position..................20
3.3
Purge the Workstation Pumps ..................................................21
3.4
Manual Control of the Pumps ...................................................21
3.5
Flush the System Through to the Fraction Collector ..................21
BioFrac Fraction Collection
3.6
Turn on the UV Lamp................................................................22
DuoFlow UV Detector
QuadTec UV/Vis Detector
3.7 pH
Electrode
Calibration ...........................................................23
3.8
Manual Screen Chromatogram Window ...................................23
3.9 Status
Bar ................................................................................24
Section 4. Anion Exchange Separation of Protein Standards ......24
4.1
Overview of the Procedure........................................................24
4.2 Prepare
Solutions .....................................................................25
4.3 Prepare
Sample ........................................................................26
4.4
Install the UNO Q1 Column.......................................................26
4.5
Prime the Pumps and Equilibrate the UNO Q1 Column.............27
4.6
Create a New Method...............................................................29
Section 5. Ordering Information.....................................................36
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