Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 38)

35
Advance
Frac. Collector
Divert Valve
1 3 2 4 5 6 7 8 9 10 11 12 13 14
100.0% Buffer B
-0.0
UV Detector
Set
UV Range
Zero
Baseline
Event
mark
Protocol: > 1 0.00 Collection Fractions of size 2.00 ml during entire run
00:00:00 00:02:00 00:04:00 00:06:00
Hr:Min:Sec AU mS/cm
Collect
Waste
Grad. Pump
Chart Recorder
700
High psi
0
Low psi
QuadTec
Zero
Baseline
1.0
0.0
100.0
75.0
50.0
25.0
Fractions
Conductivity UV
Full View
0.100
0.075
0.050
-50.0
0.025
-0.000
BioLogic Duo-Flow - Demo Chromatography with Buffe... - Standard UV Detector with Buffer Blending - Sample Run
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Window Help
Settings
WL1 - 280nm
0.2219 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
Run Time
0:00.0
Run Volume
0.0 ml
Step Time Left Fraction Vol. Left Valve Info
0.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV
0.15 AU 1.15 AU 0.30 AU
Conductivity
1 psi 0.2578 AU 3.62 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.000 Volt 6.53 pH
Edit
Bio-Rad
Web
WL2 - 260nm WL3 - 214nm WL4 - 405nm
Fig. 12. Run screen (QuadTec UV/Vis detector traces)
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