Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 8)

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1.1 Prime the Workstation Pumps
a. Immerse the workstation pump A and B inlet lines in a container of HPLC
grade (filtered, degassed) or other high quality water.
b. Connect the syringe (supplied with the fittings kit) to the priming port of
pump A.
c. Turn the priming port counter-clockwise one full turn to open the seal. Gently
withdraw the syringe plunger to draw water into the pump head.
d. Repeat this operation several times until no air bubbles are visible in the inlet
tubing.
e. Tighten the priming port by turning it clockwise.
f. Repeat this priming procedure for pump B.
1.2 Move the AVR7-3 Inject Valve to the Purge Position
Prior to purging the pumps at 10 ml/min it is essential to place the AVR7-3 valve
in the purge position. This directs the flow to waste and not to the column and
detector.
To change the position of the AVR7-3 inject valve, select P from the Manual
screen valve control panel for the AVR7-3 valve. If you plugged the AVR7-3 inject
valve into port 4 on the workstation rear panel, you will see a valve box
designated AVR7-3 at port 4. The three buttons of this box correspond to valve
positions as follows: L = Load position, I = Inject position, P = Purge position. To
move the AVR7-3 valve to Purge position, click button P.
The default position at power up and at the end of a programmed method for the
AVR7-3 is L. For all other automated valves the default is position 1.
1.3 Purge the Workstation Pumps
a. Make sure that the AVR7-3 inject valve is in the Purge position.
b. Press the Purge buttons A then B on the front of the workstation. The
workstation pumps will run at a default flow rate of 10 ml/min and the
indicator light will flash green.
c. Run each pump for 2 minutes. Press the purge buttons again to stop the pump.
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