Fig. 9. setup editor – Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
Page 32
29
c.
Equilibrate the column with 13 ml (10 column volumes) of pH 8.10 Tris (0% B).
When finished, the conductivity monitor on the status bar should read
≤ 3 mS/cm.
4.6 Create a New Method
In the Manual screen select the Browser icon from the tool bar. In the Browser
screen you will enter a user name and name for your method (refer to page 6-1
of the DuoFlow instruction manual for more information on the Browser screen)
according to the following steps:
•
Select the Browser icon from the tool bar menu
•
Select the New icon from the upper left side of the Browser screen
•
Select New from the drop-down menu and enter your user name in the dialog
box
•
Click on the Project icon for your user name
•
Select New and New Method. Enter your method name (or use default
Method 1)
•
Click OK to proceed to the instrument/devices Setup screen
Devices in setup
UV Detector
Buffer blender: Tris (25mM)
Signal Import Module 1 pH Range: 0.00 to 14.00 pH
BioFrac Fraction Collector, Rack: F1 (12-13 mm tubes)
SV5-4 Valve - Inlet A
SV5-4 Valve - Inlet B
Conductivity Monitor
AVR7-3 Valve - Sample Inject
Port 10
Available Devices
Maximizer + Gradient Pump: F10
SVT3-2
Valve
AVR7-3
Valve
SV5-4
Valve
AVR9-8
Valve
Detectors
Fraction
Collector
SV3
INJECT
P
U
M
P
Aux Load
Pump
Inlet A1:
Inlet B1:
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
Edit
Method
New
Method
New
Run
Browser
Manual Setup
Delete
Protocol
wash
load
1
2
Run
Notes
Report
PostRun
Log
Settings
Port 1
Port 2
WL1 - 280nm
0.40 AU
WL2 - 260nm
0.15 AU
WL3 - 214nm
1.15 AU
WL4 - 405nm
0.30 AU
Flow Rate
EGP %B
0 %B
0.00 ml/min
0%
% Split
1.00ml/min
0 %B2
Gradient Pump: F10
UV
Conductivity
438 psi
1.003 AU
1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH
SIM1/SIG
0.548 Volt
7.00 pH
File
View Utilities Options Window Help
Edit
Bio-Rad
Web
Buffer Blender
Is assigned to buffer blender
Is assigned to buffer blender
Inlet A2:
Inlet B2:
Is assigned to buffer blender
Is assigned to buffer blender
A1: 50 mM Tris-HCl; A2: 50 mM Tris
B1: Water; B2: 2.0M NaCl
Fig. 9. Setup editor
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