Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 16)

13
Isocratic
Flow
Add Step
Linear
Gradient
Load/Inject
Sample
Change
Valve
Hold
Alarm
Zero
Baseline
EGP
Pause
Fraction
Collection
1
2
3
4
5
0.00
0.00
1.00
1.00
Collection Fractions of size 2.00 ml during entire run
Isocratic Flow
1.50
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI 100%
0%
Volume: 1.00 ml
Flow: 4.00 ml/min
Linear Gradient
100%
100%
0%
100%
0%
0%
Flow: 4.00 ml/min
6
100% --> 50%
0% --> 50%
7
2.30
Isocratic Flow
15.30 Isocratic Flow
8 18.10 Isocratic Flow
Load/Inject Sample
Zero Baseline UV Detector
Static Loop
Sample
Auto Inject Valve
Volume: 0.50 ml
Flow: 4.00 ml/min
Volume: 0.80 ml A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
Flow: 4.00 ml/min
Volume: 13.00 ml
Flow: 4.00 ml/min
Volume: 2.80 ml
Flow: 4.00 ml/min
Volume: 8.00 ml
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
V
Column
Switching
Repeat
Steps
WL1 - 280nm
0.40 AU
WL2 - 260nm
0.15 AU
WL3 - 214nm
1.15 AU
WL4 - 405nm
0.30 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
BioLogic DuoFlow - <user name> - <project name> - <method name> - <run name>
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Window Help
Settings Cut Copy Paste Delete Edit
Edit
Bio-Rad
Web
Lamp
26.10 End of Protocol
Fig. 4. Protocol screen
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