Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
Page 36
33
The Run Screen
a.
The toolbar buttons on the left side of the screen enable you to check that
the screen display ranges for UV, QuadTec UV/Vis, pH, and conductivity are
correctly set (see page 24) and that the workstation pump pressure limits are
appropriate (700 psi high and 20 psi low limit) for the
UNO Q1 column.
b.
If you have been equilibrating the column while writing the method, you will
notice that the Status Bar is displaying the flow rate and values for UV,
QuadTec UV/Vis, and conductivity. If necessary, you may zero the UV or
QuadTec UV/Vis trace by clicking on the Zero baseline button on the
appropriate box. This button may be selected at any time.
c.
To scale the on-screen chromatogram trace display axes, use the scroll bars
located on the left and right axes of the chromatogram window.
d. To enlarge the view select the Resize button to the right of the
chromatogram display.
Start the Run
a.
Ensure that sufficient tubes are in the fraction collector rack (approximately
14). The drophead will automatically move to tube 1 when the Run is started.
b.
Ensure that the AVR7-3 valve is in the LOAD position (position L). If it is not,
return to the Manual screen by clicking the toolbar Manual button and click
on valve position L.
c.
Ensure that the 50 µl sample loop is connected to ports 3 and 6 of the inject
valve. Completely fill the loop with protein standard via port 5 and a syringe
and needle. Do not remove the syringe from the injection port after filling the
loop or the sample will siphon to waste.
d.
To launch the Run, click on the green Start toolbar button. The sample will
be loaded automatically.
e.
When the run is finished, the pumps automatically stop and a “Run Finished”
message appears in the bottom right of the status bar.
f.
Figures 12 and 13 show typical run screens and chromatograms for this
separation using the UV detector or QuadTec UV/Vis detector.
4006208B.qxd 6/28/2004 11:04 AM Page 38