Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 17)

Protein SmartSpec Plus will automatically read one or more
wavelengths depending on the type of assay specified
(Bradford, Lowry, BCA or UV Protein) or the user
can specify a wavelength. SmartSpec Plus will
convert absorbance data to concentration by use of a
standard curve, except for the case of UV Protein in
which the absorbance readings are multiplied by
user-selectable constants. The standard curve can be
one recalled from memory, or a new one constructed
by SmartSpec Plus.
Scan SmartSpec Plus will collect and print the absorbance
of a sample over a user-defined range of wavelengths
from 200 to 800 nm. An optional background
wavelength may be specified and its absorbance will
be subtracted from all absorbances in the scan range
before the scan is displayed. SmartSpec Plus will
identify and quantitate the points of maximum and
minimum absorbance over the scanned range. These
data can be printed or downloaded via the serial port
to an external computer.
Kinetics SmartSpec Plus will collect data from a single cuvette
at fixed periodic intervals over a certain time period.
Both the interval of data collection and length of data
collection are specified by the user. An optional
background wavelength may be specified. The kinetic
readings may be printed or exported to external
software for analysis.
OD600 SmartSpec Plus will collect absorbance data at 600
nm. This assay is used for monitoring the growth of
bacterial cultures. The user may specify a conversion
factor which is multiplied by the dilution factor and
the absorbance to calculate the concentration in cells
per ml.
λλ SmartSpec Plus will collect data at up to three different
user-specified wavelengths. An optional background
wavelength may also be specified and its absorbance
will be subtracted from the other wavelengths before
they are reported.
Read Blank SmartSpec Plus will zero the detector in preparation for
reading samples. It is important to zero the instrument
at each new assay. Insert a cuvette that contains all
assay components except the analyte and press Read
Blank.
Read Sample SmartSpec Plus will collect absorbance data from the
cuvette in the instrument.
Select Used to toggle between or among choices. Once the
choice is made, its selection is confirmed by pressing
Enter.
Enter Indicates to SmartSpec Plus that a choice has been
made.
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