Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 46)

If you want to rescale the Y axis, press Enter. If you want to rescale the X axis, press
Select to toggle the display and press Enter.
When you rescale the X axis, you must input the new maximum X value and press
Enter. A new maximum Y value is automatically calculated and the graph is plotted.
When you rescale the Y axis, you must input the new maximum Y value and press
Enter. A new maximum X value is automatically calculated and the graph is plotted.
Quantitating UV-proteins:
Protein (mg/ml) = C1 * (A
280
– A
320
) – C2 * (A
260
-A
320
)
Coefficients 1 and 2 (defaults 1.55 and 0.76, respectively) can be changed for different pro-
teins.
43
Max conc=150.0000
Enter new max:
Max abs=1.234
Enter new max:
Rescale standard curve?
Absorbance (X-axis)