Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 48)

• Use the number keys to input the number of successive scans and press Enter.
SmartSpec Plus will now display the Ready screen. If you want to invoke the smoothing
option or the subregion option, choose Options from the Ready screen.
The first three options allow you to change the conditions already specified above. Press
the right arrow key to see the other options.
If you choose Regions, SmartSpec Plus will prompt for the number of regions.
• Use the number keys to input the number of regions. Choose 0 if you don't want
any subregions. If you specify 1 or more subregions, then you are next prompted to
define them.
• Use the number keys to input the beginning wavelength and press Enter. Then use
the number keys to input the ending wavelength for the first subregion and press
Enter again. After you define the last subregion, the Option screen is displayed
again.
• To turn smoothing on, press 5.
• Press Select to toggle from Off to On and press Enter.
45
Ready to read absorbance
<=Exit Assay >=Options
1. Repetitions 2. Range
3. Background >=More
Enter number of
subregions (0–4):
Subregion #1:
Start: nm. End: nm
Select Smoothing: Off
4. Subregions 5. Smooth
<=Back, Cancel = return
4. Subregions 5. Smooth
<=Back, Cancel = return