Bio-Rad SmartSpec Plus Spectrophotometer User Manual
Page 2
Table of Contents
Page
Section 1 General Safety Information .....................................................................1
1.1 Caution
Symbol......................................................................................................1
1.2 Precautions.............................................................................................................1
Section 2 Packing List ..............................................................................................1
Section 3 Installation ................................................................................................2
3.1 Environmental
Requirements .................................................................................2
3.2
Power On and Initial Display .................................................................................2
Section 4 Introduction ..............................................................................................3
4.1 Identification
of
System Components.....................................................................3
4.2 Setup ......................................................................................................................7
4.3 Serial
Port
Functions ............................................................................................10
4.4
PC Serial Connection Instructions........................................................................11
4.5 Key
Pad
Descriptions ...........................................................................................13
Section 5 Quick Guide............................................................................................15
Section 6 Operating Instructions ...........................................................................17
Section 7 Examples of Assays.................................................................................25
7.1 DNA/RNA ...........................................................................................................25
7.2 Protein
Assay .......................................................................................................31
7.3 Spectral
Scanning.................................................................................................44
7.4 Kinetics ................................................................................................................46
7.5 OD600..................................................................................................................47
7.6 Lambda
(
λ
) ..........................................................................................................48
Section 8 Troubleshooting ......................................................................................49
Section 9 Maintenance............................................................................................50
9.1
Cleaning Up a Fluid Spill .....................................................................................50
9.2 Cleaning
Quartz
Cuvettes.....................................................................................51
9.3
Changing Thermal Printer Paper ..........................................................................51
Section 10 Specifications ..........................................................................................52
Section 11 System, Accessories, and Reagents for SmartSpec
Spectrophotometer .................................................................................53
11.1 Product
Information .............................................................................................53
11.2 Bio-Rad
Protein
Assays........................................................................................53
11.3 Cuvette
Selection
Guide.......................................................................................54
11.4
Selecting the Appropriate Cuvette........................................................................54
For technical service call your local Bio-Rad office. In the U.S., call 1-800-4BIORAD
(1-800-424-6723).
Copyright© (2002) Bio-Rad Laboratories, Inc. All rights reserved.