Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 45)

• Press 0 to see the intercept. Press the right arrow to return to the assay or press the
left arrow key to see the slope displayed again.
If the slope or intercept has a value greater than 9999 or less than 0.0001, it will be shown
in exponential notation. In all cases with SmartSpec Plus, e represents the base 10, not the
base of the natural logarithm. For example, 100,000 would be represented as 1.000 e 05
and 0.00001 would be represented as 1.000 e-05.
• Press 0 to see the creation date of the standard curve.
• From this display, press the left arrow to see the intercept again, or press the right
arrow to return to the assay.
• When you return to the assay, SmartSpec Plus will be ready to collect absorbance
data and to covert the absorbances into concentrations using the new standard curve.
E. Printing Standard Curves. If you choose to print a full report at the end of an assay,
the standard curve will be printed along with the information about the curve, including
the concentrations and absorbances of the standards, and the slope, intercept and correlation
coefficient of the curve.
You can choose to print the same information about a standard curve when it is first
created or when it is recalled from memory. When a standard curve is newly created the
following prompt is displayed:
Press Select to toggle from No to Yes and press Enter. Now you can press Print to
begin printing the standard curve. First the printer is initialized and then you are offered
the choice of printing just the graph, just the data used to create the graph, or all the
information.
If you choose to print both the plot and the data, the data are first printed and then you
are offered a chance to rescale the axes of the plot. You may choose to rescale the Y
(absorbance) or the X (concentration) axis, and the other axis will be automatically
rescaled at the same time.
If you wish to rescale the axes, press Select to toggle from No to Yes and press Enter.
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Name: 11/25/98
<=Back >=Continue
Ready to read absorbance
<=Exit Assay >=Options
Want to view info on new
STD CURVE now? No >=HELP
Print: 1=Data only
2=Plot only 3=both
Rescale standard curve?
NO
Rescale standard curve?
Absorbance (Y-axis)
Intercept (I)=xx.xx e xx
<=Back 0=More >=Continue