Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 41)

b. If there are varying numbers of standard replicates. Each time SmartSpec Plus
prepares to read a standard, you will be prompted for the number of replicates for that
standard and then for the concentration of the standard. After all replicates of the first
standard are read, then SmartSpec Plus will prompt for the number of replicates of the
second standard and then for the concentration of the second standard. This will contin-
ue until the last replicate of the last standard is read.
• Use the number keys to input the number of replicates of standard #1 and press Enter.
Now SmartSpec Plus will prompt for the concentration of the first standard and then
for units of concentration.
• Use the number keys to input the concentration of standard #1 and press Enter.
You may enter concentrations with as many as eight significant digits, but all results
are reported with four significant digits because SmartSpec Plus absorbance data are
measured to four significant digits.
SmartSpec Plus will begin to collect absorbance measurements on the replicates of
standard #1.
• Insert the cuvette with the first replicate of standard #1 into SmartSpec Plus and
press Read Sample.
SmartSpec Plus will continue to prompt for each additional replicate of standard #1
until all are read.
•
•
•
38
Enter number replicates
for Standard #1 (1–9): __
Enter concentration of
Standard #1: _________
Insert Rep #1 of Std #1
and press Read Sample
Insert Rep #2 of Std #1
and press Read Sample
Insert Rep #9 of Std #1
and press Read Sample