Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 42)

After all replicates of standard #1 are measured, SmartSpec Plus asks for the number of
replicates of standard #2 and then the concentration of standard #2 before prompting
you to insert each of the replicates of standard #2.
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When collecting data for construction of the standard curve, SmartSpec Plus does not
display the absorbance data, but you may view it by pressing Abs. If you press Abs, you
must indicate if you want the reading of the last replicate or if you want the mean and
standard deviation of the current replicate group. All standard absorbance data can also
be printed at the end of the assay.
• Press the left arrow key to view the absorbance of the last replicate.
or
• Press the right arrow key to view the mean and standard deviation of the absorbance
of current replicate group.
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<=Show last replicate
>=Show mean and std dev
Insert Rep #9 of Std #9
and press Read Sample
Enter number replicates
for Standard #2 (1–9): __
Enter concentration of
Standard #2: ________
Insert Rep #1 of Std #2
and press Read Sample
Insert Rep #5 of Std #2
and press Read Sample
Enter number replicates
for Standard #9 (1–9): __
Enter concentration of
Standard #9: _________
Insert Rep #1 of Std #9
and press Read Sample