Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 24)

• Press the right arrow key to change the number of sample replicates.
B. Sample replicates.
•
Place the first replicate of your first sample in the cuvette chamber and press Read
Sample. Before it collects the absorbance data, SmartSpec Plus will ask for the
number of replicates for the first sample.
• Use the number keys to input the number of replicates and press Enter. The
absorbance data for the first replicate will be collected and the SmartSpec Plus will
direct you to insert the second replicate
This will continue until the last replicate of the first sample has been read. SmartSpec
Plus will then display the mean absorbance of all replicates of the first sample as
described below. When the absorbance data are displayed, you can insert the first repli-
cate of the second sample and press Read Sample.
•
You can change the number of sample replicates at any time in the assay by first
going to the Ready screen (if absorbance data are displayed, you can return to the
Ready screen by pressing Enter).
• Press the right arrow for Options.
• Press the right arrow key to change the number of sample replicates.
• Use the number keys to input the new number of sample replicates and press
Enter.
6. View the absorbance and concentration data. Absorbance data are automatically displayed
after they are collected. In the case that you are reading samples in replicate groups, the mean of
the replicate group is displayed. The standard deviation of the replicate group is available in the
printed report.
A. DNA/RNA. Immediately after the sample is read, the data are displayed in the
form
• Press Abs to toggle the display to view the other absorbance data
21
A280= x.xxx A320= x.xxx
Conc = xxx.xxx µg/ml
A260= x.xxx Samp #1
Conc= xxx.xxx µg/ml
Enter number of sample
replicates: ______
Ready to Read absorbance
<=Exit Assay >=Options
Sample #1 Enter number
of replicates: 3 >=Option
Insert Rep #2/3 Samp #1
and press Read Sample
<=Change next sample #
>=Change # of replicates
Enter number of sample
replicates: _____