Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 35)

3. Standard Curves. Once you have selected the type of assay, SmartSpec Plus will ask
if you want to make a new standard curve. You can decline to make a standard curve,
in which case it will not be possible for SmartSpec Plus to convert absorbance readings
to concentration; you can recall a saved standard curve from memory, or you can create
a new standard curve.
If you want to make a new standard curve, press Enter and skip ahead to part B below.
If you do not want to make a new standard curve, press Select to toggle from Yes to
No and press Enter.
A. If you choose not to create a new standard curve. SmartSpec Plus will offer you
the opportunity to recall a standard curve from memory.
• If you want to recall a stored standard curve, press Enter and skip ahead to part ii
below. If you don’t want to recall a standard curve from memory, press Select to
toggle from Yes to No and press Enter.
i. If you do not recall a standard curve from memory. SmartSpec Plus will
alert you that it will not be able to convert absorbance readings into
concentrations.
• If you want SmartSpec Plus to convert absorbance to concentration, press
Select to toggle from Yes to No and press Enter. You will be prompted
again to create a new standard curve. If you don’t mind that concentrations
cannot be calculated, press Enter and SmartSpec Plus will be ready to collect
absorbance data.
ii. If you want to recall a stored standard curve from memory.
• Press Select to scroll through the list of stored standard curves until you see
the one you want to recall, and then press Enter.
Note that the type of assay being performed when the standard curve was calculated
is also displayed.
After you choose the standard curve, SmartSpec Plus will offer you a chance to
view the slope, intercept, correlation coefficient and date of creation for the
curve. These data will be printed on the final report.
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Do you want to recall a
stored std curve? Yes
Do you want to make a
new standard curve? Yes
Select Curve: Bradford
Std Curve #1 N A M E
Want to view info on std
curve #1 now? No >=Help
Cannot calculate conc
without std curve. OK? Yes
Ready to read absorbance
<=Exit Assay >=Options