Bio-Rad VINEO™ Brettanomytest PCR Kit - Manual (Page 5)

This test is used to detect and quantify Brettanomyces bruxellensis in the
DNA extracted from fermented drinks produced from grape and
fermenting grape musts.
3 - KIT CONTENTS
This kit contains the quantity of reagent required for 96 reactions.
4 - VALIDITY AND PRESERVATION
On reception, the kit must be stored at temperatures between +2°C and
+8°C. Each reagent stored between +2°C and +8°C can be used until the
expiry date indicated on the tube.
NB: Do not freeze the reagents.
5 - REQUIRED EQUIPMENT THAT IS NOT PROVIDED
Equipment:
• Industrial Diagnostic CFX96™ real-time PCR detection system, 96
wells, ref. Bio-Rad :359-3990
• CFX Manager™ Software, Industrial Diagnostic Edition, ref. Bio-Rad :
359-3893
• MiniOpticon™ system for real-time PCR, reaction block of 48 wells,
ref. Bio-Rad: 359-3995
• Vortex
• Optionally: centrifuge with rotor for plates or strips (max. 2000 x G)
• 20 μl, 200 μl and 1000 μl micropipettes
• Multi-distributor such as combitip pipettes
Note: We recommend using an uninterruptible power supply with the
thermocycler.
5
© 2010 Bio-Rad
Stoppers Designation Reagent Volume
Pink Fluorescent probes Fluorescent probes 1 tube (0.55 mL)
White Amplification mix Amplification mix 2 tubes (2 x 2.2 mL)
Green Negative PCR control Negative PCR control 1 tube (0.5 mL)
Red Positive Qs PCR control Positive Qs PCR control 1 tube (0.5 mL)
TAKING
THE SAMPLE
DNA EXTRACTION
VINEO™ Extract
DNA Kit
(Ref.: 354-8100)
DNA
AMPLIFICATION by
PCR IN REAL TIME
VINEO™
Brettanomytest PCR
Kit (Ref.: 354-8101)
ANALYSIS &
INTERPRETATION
Opticon Monitor™
or
CFX Manager™ IDE