Bio-Rad VINEO™ Brettanomytest PCR Kit - Manual (Page 6)

Consumables:
• For the plates, PCR tubes and stop caps:
- White plates of 96 "Low-profile" wells, ref. Bio-Rad: MLL-9651
- White plates of 48 "Low-profile" wells, ref. Bio-Rad: MLL-4851
- Strips of 8 tubes of 200μl "Low-profile", ref. Bio-Rad: TLS-0851
- Strips of 8 flat optical stop caps for tubes or plates of 0.2 mL wells,
120 strips ref. Bio-Rad: TCS-0803
• Cones for Combitip or multi-distributor, sterile when packaged
individually
• Cones with sterile filters that are adaptable to 10 μL, 20 μL, 100 μL,
200 μL and 1000 μL micro-pipettes
• 2mL and 5mL sterile tubes
• Non-powdered latex or nitrile gloves
• Sterile distilled water
• 5% Chlorine Bleach
• Decontaminating agent such as DNA AWAY
® or RNAse AWAY ®
6 - PRECAUTIONS AND RECOMMENDATIONS
• This trial must be carried out by staff who have had adequate
training.
• Result quality depends on scrupulous compliance with Laboratory
Good Practice, in particular in the area of PCR:
- The equipment (pipettes, tubes etc.) must no go from one work
station to another.
- It is indispensable to use a positive and a negative control for each
series of amplification reactions.
- Do not use the reagents beyond their expiry date.
- Subject the reagents in the kit to a vortex movement using the
apparatus provided for this purpose before using them to work
with homogeneous solutions.
- Check the exactness and the precision of the pipettes as well as
the proper functioning of the instruments.
- Change gloves regularly and as soon as you suspect that they may
have been contaminated.
- Clean work surfaces regularly with a 5% solution of chlorine bleach
and another agent such as DNA AWAY ® or RNase AWAY ® .
- Wear non-powdered gloves so as not to leave fingerprints on the
optical film used to seal the micro-plates. Do not write on the PCR
tube stoppers. In both cases, recording the data by the device can
be disturbed.
6
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