Metrohm 844 UV/VIS Compact IC - Manual (Page 46)

Table of contents
4 Deaerate pump
• Make sure that the aspirating tubing 82 for the high-pressure
pump has been immersed into the eluent.
• Click the <On> button for IC pump to switch on the high-pres-
sure pump.
• Use the syringe inserted into connection 6 to aspirate air until
eluent flows bubble-free into the syringe.
• Click the <Off> button for IC pump to switch off the high-pres-
sure pump.
• Close the rotary knob on purge valve 32 by turning it in a
clockwise direction.
• Remove the syringe from connection 6.
5 Rinse pulsation dampener
• Place a beaker beneath the column connection capillary 47.
• Click the
<On> button for IC pump to switch on the high-
pressure pump and rinse the pulsation dampener 36 filled
with isopropanol for approx. 10 min with eluent.
• Click the
<Off> button for IC pump to switch off the high-pres-
sure pump.
6 Reduce flow rate
• Reset the original flow rate under
Flow (e.g. 0.5 mL/min ).
• Click
<Set> to send this value to the 844 UV/VIS Compact IC.
• Click
<Save> to save the values.
2.7 Post column reactor
This chapter covers the two versions 2.844.0020 and
2.844.0120.
With versions 2.844.0010, 2.844.0030, 2.844.0110 or
2.844.0130 you can skip this chapter and go directly to Section
2.8.
Instrument versions 2.844.0020 and 2.844.0120 of the 844 UV/VIS
Compact IC contain the 6.2836.000 Post column reactor (see
Instructions for Use 8.108.1031 Post column reactor).
2.7.1 Preparing the peristaltic pump
Before it is started up, the accessories must be mounted on the
peristaltic pump built into the 844 UV/VIS Compact IC Proceed as
follows:
1 Remove the tubing cartridge
• Loosen tubing cartridge 96 mounted above pump drive 51 by
pressing down snap-action lever 98 from holder clip 50 and
remove it from mounting pin 40.