Create a new batch from an existing protocol, Create a new batch from a new protocol, Create a new – Luminex xPONENT 3.1 Rev 2 User Manual
Page 114: Batch from an existing protocol
Create a New Batch from an existing Protocol
Read the instructions provided with the assay kit you are using.
1. Open the Batches page.
2. Click Create a New Batch from an existing Protocol.
3. Type the batch name in the Batch Name box.
4. Type a description about the batch in the Enter Optional Description box.
5. Click a protocol you wish to use in the Select a Protocol list.
6. Click Next. If the protocol uses standards, controls, or both, the next tab that appears is
the Stds & Ctrls tab. View the details of the active reagents or apply different assay
standards, controls, or both or manually enter new information. Select Next. If the
selected protocol does not use standards, controls, or both, the next tab that appears is
the Plate Layout tab.
7. On the Plate Layout tab, assign well commands for this batch. See
a complete description of the commands and options on this tab.
8. Click Run Batch to begin batch acquisition, or click Save to save batch information to the
Pending Batch list to be run at a later time.
NOTE: If the batch spans more than one plate, the tray ejects automatically
when all defined wells have been acquired. A dialog box opens,
prompting you to insert the next plate.
Create a New Batch from a New Protocol
This option creates a new batch to acquire data without using an existing protocol. It writes
raw data results to a simple ".csv" file format. You can define parameters for samples, gates,
regions, events, and commands.
For 96-well plates, use plates with wells that will hold at least 190 μL (the extra 25 μL from
the sample, plus an extra 165 μL that are dispensed back into the well following acquisition).
1. Open the Batches page.
2. Click Create a New Batch from a new Protocol to open the Settings tab.
3. Type a name in the Name box.
4. Type a description in the Description box.
5. Define the settings in the Acquisition Settings section.
NOTE: For information about each of these settings, see the
section.
6. Define the settings in the Analysis Settings section. For more information about each of
these settings, see the Analysis Settings information within the
section. If
you select Quantitative, Allele Call or Qualitative from the Analysis Type list, you can
select Analyze results while acquiring samples to view real-time analysis. The
xPONENT Analysis Package can only analyze batches acquired using qualitative,
quantitative, or allele call protocols.
7. Click Next. The Analytes tab opens.
8. Click the desired analyte in the numbered grid. Information about the analyte displays in
a list on the right side of the grid.
Using the Software
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