Luminex xPONENT 3.1 Rev 2 - Manual (Page 122)

Creating a Qualitative Assay Protocol
You define each Qualitative assay protocol using a single kit. The kit must contain one
standard. The standard is assigned a “Quali” value for each test. Controls are optional, but
are recommended for judging the acceptability of batch results.
To create a Qualitative Assay Protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Settings tab opens.
2. In the Name box, type the name of the protocol.
3. In the Version box, type the version of the protocol.
4. In the Manufacturer box, type the manufacturer information for the protocol.
5. Define settings in the Acquisition Settings section.
6. Define settings in the Analysis Settings section, selecting Qualitative as the analysis
type. The protocol for a qualitative assay contains a single standard.
7. Click Next. The Analytes tab opens.
8. Click the desired analytes (bead IDs) in the numbered grid. Information about the analyte
displays in a list on the right side of the grid.
9. To change the Default Analysis, click Change. The Analysis Settings dialog box
opens.
10. In the Analysis Settings dialog box, click the analysis method from the Analysis
Method list. Click OK to change the default analysis to a single analyte. Click Apply to
All Analytes to apply the selection to all analytes. Skip this step if you do not want to
change the default analysis or if you chose No Analysis on the Settings tab.
11. In the Units box, type the desired unit of measurement.
12. In the Count box, type the desired bead count for each analyte. Click Apply All.
Using the Software
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