Luminex xPONENT 3.1 Rev 2 - Manual (Page 119)

9. On the Stds & Ctrls tab, type the appropriate information in the Standard Information
and Control Information sections. The number of Standards and/or Controls in these
sections is defined on the Settings tab in the Analysis Settings section. If your batch
uses controls, enter the appropriate values for Expected Values. Click Low Value from
the Show list, and enter the low value for each analyte. Click High Value from the Show
list, and enter the high value for each analyte. Reagent information is not required to
replay a custom batch, unless you want to use the analysis feature.
10. Click Next.
11. In the Plate Layout tab, you can do the following:
• To add well commands, choose the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you wish to run in replicate
mode, change the Replicate Count to the appropriate number and the Grouping to
your preferred grouping method.
• To add maintenance commands, choose the desired command from the list. Choose
the well that you want to apply it to, and then choose Before Well or After Well. If you
are working with more than one plate, choose the Add & Change Plate drop down list.
Here, you can add a plate, change the order of the plates, and scroll through all plates.
• As you add commands to your plate, they appear in the command sequence list. Here,
you can give each of your wells an ID. You can also import an ID list and move your
commands up and down in the command sequence chart.
NOTE: Only wells that were acquired in the original batch can be replayed.
12. Click Replay Batch. The Current Batch tab opens, where you can view the data as it is
replayed.
Analyze Saved Batch
Click Results on the xPONENT toolbar, then open the Saved Batches tab. Click the batch
name, then click Open. The Results, Settings, Log, and Sample Details tabs appear.
• Click the Results tab to view statistical information about the batch. See Sample Details
Tab .
• Click the Settings tab to view the batch settings report. See Settings Tab
• Click the Log tab to view a log for the activity that occurred during acquisition of the
selected batch. See Log Tab .
• Click the Sample Details tab to view sample details for each sample in the batch. If you
are using the LIS package, click Transmit to transmit the data to the LIS.
Validate Standards
Your xPONENT ® system administrator must give you privileges to validate standards if you
are using the Secure xPONENT package. All standards are assumed to be valid unless
explicitly invalidated.
1. Open the Results page.
2. Open the Saved Batches tab.
3. Click the batch name, then click Open. The Results tab opens.
4. Click the square area next to left of the standard you wish to validate, then click Validate.
xPONENT ® 3.1 Rev. 2
106