Luminex xPONENT 3.1 Rev 2 - Manual (Page 127)

NOTE: For more information about the commands and settings on the Plate
Layout tab, see the Plate Layout Tab .
Editing Quantitative Analysis Settings
To edit quantitative analysis settings:
1. Open the Protocols page, then open the Protocols tab. Select a protocol and click Edit.
2. From the Analytes tab, click an analyte’s Analysis field to open the Analysis Settings
dialog box.
3. From the Method list, click No Analysis, Cubic Spline, Linear Fit, Logistic 4P, or
Logistic 5P.
4. From the Weight list, click None or 1/Y ^2 .
NOTE: The Weight options are only available if the chosen Method is
Logistic 4P or Logistic 5P.
5. Check Mark as Intra-Well Normalization Bead if you want to use a normalization bead.
The normalization bead is a microsphere set that is included in the assay as an internal
control. It controls for sample variation and can be used to normalize data between
samples in a run.
6. Check Use Threshold Ranges if you want to use a range for the analysis.
7. Click Add Range to setup the threshold range.
8. Type a name for the range in the Range Name field.
9. Type low and high range values in the Low Value and High Value fields.
10. Select the check box in the Inclusive column to include the value in the range, or leave it
clear to make the range value one unit higher than the low value, and one unit lower than
the high value.
11. Highlight a range and click Delete Range to delete the range.
12. Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes to
apply them to all the analytes in the protocol.
Creating an Allele Call Protocol
This protocol contains no standards or controls. An allele call protocol compares groups of
two, three, or four analytes to identify genotypes.
To create an allele call protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Protocol Settings tab opens.
2. In the Name box, type the name of the protocol.
3. In the Description box, type a description of the protocol.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define the settings in the Acquisition Settings section.
7. Define settings in the Analysis Settings section, selecting Allele Call as the analysis
type.
8. Click Next. The Analytes tab opens.
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