Luminex xPONENT 3.1 Rev 2 - Manual (Page 17)

medical advice immediately and show the product label or container to your medical provider.
A Material Safety Data Sheet is available upon request.
WARNING: Reagents may contain sodium azide as a preservative. Sodium
azide may react with lead and copper plumbing to form highly
explosive metal azides. It is also highly toxic and rates a
material safety data sheet (MSDS) health hazard 4. On
disposal, flush drains with a generous amount of cold water to
prevent azide build-up. Consult the manual guideline “Safety
Management No. CDC-22, Decontamination of Laboratory Sink
Drains to remove Azide salts” (Centers for Disease Control,
Atlanta, Georgia, April 30, 1976).
CAUTION: The sheath fluid and the solution in which beads are stored
contain ProClin
® , which may cause an allergic reaction. Use
personal protective equipment, including gloves and safety
glasses.
Bead Concentration
The concentration of beads in an assay is a factor in system speed. If running an xMAP-
based kit, follow the instruction found on the kit’s product insert or use the provided software
protocol. We generally recommend using 2000 to 5000 beads per target, depending on the
assay.
Repetitive xMAP Bead Measurements
In an xMAP assay, the reporter signal is the result of the assay. Due to small bead size,
xMAP bead suspension exhibits near solution phase reaction kinetics. This means that each
set of xMAP beads used for a particular assay will show a statistically even distribution of
reporter molecules bound to the surface of each bead. During data acquisition, numerous
beads of each set are analyzed and the median statistic is computed for that set by the
software. The more beads of a set measured, the more confidence that can be given for that
particular measurement. If running an xMAP-based kit, follow the kit’s product insert or use
the provided software protocol.
Classification and Reporter Fluorochromes
Each xMAP bead set is internally dyed with two classification dyes. The fluorescence signal
of these dyes allows for classification of each bead set. Since each bead is analyzed
individually, even when the sets are mixed in a multiplex assay they can still be distinguished
by their emission signals. The fluorescence signal of reporter molecules bound to the surface
of each bead set is measured and used to determine the result of each assay in a multiplex.
Again, since each bead is analyzed individually, reporter signals for each bead set can be
accurately quantified.
The following table displays acceptable reporter fluorochromes and their excitation and
emission wavelengths.
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