Luminex xPONENT 3.1 Rev 2 - Manual (Page 123)

13. Click Next. The Plate Layout tab opens.
• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the grouping to your
preferred grouping method.
• To add maintenance commands, choose a command from the list. Highlight the well
that you want to apply it to, and then choose Pre Batch Routine or Post Batch
Routine. The Commands and Routines dialog box opens.
If you are working with more than one plate, choose Add & Change Plate. Here, you
can add a plate, change the order of the plates, and scroll through all plates.
14. Click Save.
Editing Qualitative Analysis Settings
1. Open the Protocols page, then open the Protocols tab. Select a protocol and click Edit.
2. On the Analytes tab, click an analyte's Analysis field on the right side of the window to
open the Analysis Settings dialog box.
3. From the Method list, click Luminex Qualitative or No Analysis.
4. From the Formulas list, click Lum Qual or Adv Qual.
5. Type a new name for the edited formula in the Formula Name box.
6. If you selected Lum Qual in the Formulas list, edit the values for each range under
Negative, Low Positive, or High Positive. If you selected Adv Qual in the Formulas
list, edit Negative, Low Positive, Moderate Positive, or Strong Positive. Select the
check box in the Inclusive column to include the value in the range. If you clear the
check box, the value will be one unit higher than the low value, and one unit lower than
the high value.
7. Click Add Range if you want to add a new range. Type a Range Name, Low Value,
High Value, and check or clear the Inclusive check boxes.
8. If you want to delete a range, click a range to highlight it, and click Delete Range.
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