Luminex xPONENT 3.1 Rev 2 - Manual (Page 115)

9. To change the Default analysis, click Change. The Analysis Settings dialog box
opens.
10. Select the analysis method from the Method list. Click OK to change the default analysis
for analytes to be selected. Click Apply to All Analytes to apply the selection to all
analytes. The Analysis dialog box closes.
11. Type the units of measurement you want to use in the Units box. If you click Apply All,
this choice is applied to all analytes. To change individual units, change them in the
analyte table in the dialog box.
12. Type the desired bead count for each analyte in the Count box. If you want to change
individual bead counts, change them in the analyte table in the dialog box.
13. Click Next. The Stds & Ctrls tab opens . This tab does not open if no analysis is
selected.
14. If you are using an assay standard/control kit, click Apply Std/Ctrl Kit. In the Select Std/
Ctrl Kit dialog box, click the kit from the list and click OK. Applying a kit only works for
kits already installed, however, you can manually type the information.
15. If you are not using a kit, type the appropriate information in the Standard Information
and Control Information sections. The number of standards and/or controls in these
sections is defined on the Settings tab in the Analysis Settings section. If your batch
uses controls enter the high value for each analyte. Reagent information is not required
for a custom batch unless you want to use the analysis feature.
16. Click Next. The Plate Layout tab opens.
• To add well commands, click the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose the desired command from the list. Choose
the well that you want to apply it to, and then choose Before well command or After
well command. If you are working with more than one plate, choose the Plate list.
Here, you can add a plate, change the order of the plates, and scroll through all plates
• As you add commands to your plate, they appear in the Command Sequence list.
Here you can give each of your wells an ID. You can also import an ID list and move
your commands up and down to change the order in which they will be acquired. For
additional information, see the
Plate Layout Tab section.
17. Click Single Step to acquire the first two wells, then pause acquisition.
18. Click Run Batch to run the acquisition, or Save the batch for a later time.
NOTE: If the batch spans more than one plate, the tray ejects automatically
when all defined wells have been acquired. A dialog box displays
prompting you to insert the next plate.
Create a Multi-Batch
A multi-batch is a set of batches that you want to process consecutively. You add batches to
the multi-batch from pending batches in your database. You can also create a new batch to
add to the database for the multi-batch. You can include as many batches as you need. The
software does not limit you to a certain number of batches per multi-batch.
You must ensure the batches fit on one plate. After you add each batch, the software
automatically adds the next batch to the first well of the next column or row (depending on the
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