Luminex xPONENT 3.1 Rev 2 - Manual (Page 126)

27. Click Next. The Plate Layout tab opens.
• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you wish to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose a command from the list. Highlight the well
that you want to apply it to, and then choose Pre Batch Routine or Post Batch
Routine. The Commands and Routines dialog box opens.
If you are working with more than one plate, choose Add & Change Plate. Here, you
can add a plate, change the order of the plates, and scroll through all plates.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
• As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can
also import a protocol file by clicking Import List.
• Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.
28. Click Save.
NOTE: The Pre Batch routine runs before the batch begins. This routine is
selected by default when the protocol or batch is set up. The default
Pre Batch routine can be changed in the Batch Options page.
NOTE: The Post Batch routine runs after the batch is complete. This routine
is selected by default when the protocol or batch is set up. The
default Post Batch routine can be changed in the Batch Options
tab of the Admin page.
Using the Software
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