Luminex xPONENT 3.1 Rev 2 User Manual

Page 51

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Replay Batch - Uses the data stored in the run files from the initial acquisition to reprocess a
batch, creating a new batch output file. A batch can be reprocessed multiple times.

NOTE: The initial batch data and output file always remain intact and

unchanged. Each time you replay a batch, the system handles it as if
it is a new batch, creating a separate batch entry and output file.

Replay a batch to replay the raw bead data files. The bead data files will be replayed using
the gate, analyte, analysis settings, and plate layout selected in the new (or updated)
protocol. Settings such as bead type, volume, timeout, XY heater, and report gain will have
no effect.

Recalculate Data - Reanalyzes the batch results using only the batch’s MFI values.

Open - Opens the Results tab. Use this tab to view the saved batch results for the selected
batch.

The batch MFI values will be recalculated using the analysis settings and plate layout
selected in the new recalculated batch or protocol. Settings such as bead type, volume,
timeout, XY heater, gate, report, and gain will have no effect. Because only the MFI values
are reanalyzed, no data will be displayed in the dot plot or histogram.

When you replay or recalculate a batch, you perform the same steps to create the batch as
you did when you created the batch the first time. This sequence will vary based on whether
you created a new batch from a new protocol or a new batch from an existing protocol. The
functionality of the tabs you see while configuring the replay batch is the same as described
in “Batches Tab” section.

Open - Opens the Results tab. Use this tab to view the saved batch results for the selected
batch.

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3.1 Rev. 2

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