Luminex xPONENT 3.1 Rev 2 - Manual (Page 46)

Both the histogram and the dot plot have buttons that enable you to change the default view:
Click the maximize button to maximize the graph to fill the whole window. In the
maximized view, use the sliders to zoom in or zoom out. To return to the standard view,
click the minimize button.
If the histogram or dot plot display have been changed to another view (e.g. Progress,
Well Report), click Default to return to the default histogram and dot plot displays.
Histogram - The default location of the histogram is the lower left section of the Current
Batch tab. The Doublet Discriminator (DD) displays on the X axis. Events display on the Y
axis. Doublets appear when two beads stick together, creating undesired results. When you
select the gate, two vertical dashed red lines appear. These represent gate positions
determined by the protocol. Once the gate is set, everything outside of it is ignored. For
batches created using an existing protocol, the gate cannot be changed while running the
batch. For new batches created from new protocols, the gate can be changed while running
the batch. Any changes made to the gate while running new protocol batches immediately
affect the visual display of the dot plot and histogram. In addition, changes made to the gate
while running new protocol batches affect the acquisition for all wells initiated after the
change is made. When viewing saved batch data, the gate can be changed to visually
inspect the effect. Only the visual display of the dot plot and histogram is affected for saved
batches, not the acquired data.
NOTE: Gate positions are dependent upon buffer composition. Any changes
made to your assay buffer composition may result in a different
optimal gate location.
Right-click inside the histogram area to access the following options:
• X Axis - Use this function to change the X axis to the following: Doublet Discriminator,
Reporter 1, Classification 1, and Classification 2. Doublet Discriminator is the default.
NOTE: You can change the X axis of the histogram for troubleshooting
purposes. Use only the default settings in all other instances.
• Gate - Show, create, delete, or move the gate. You can also move the gates by clicking
and dragging the red lines on the histogram.
• Autoscale - Click to automatically adjust the maximum number of events shown on the Y
axis. Click during acquisition to readjust the Y axis scale.
• Set Scale - The Set Scale dialog box displays. Set the maximum number of events and
click OK. The default number of events is 60.
• View - You can choose to view all events, or only gated events.
• Display Mode - Opens the Histogram Options dialog box. Click either Logarithmic or
Linear display. Logarithmic display is the default. Select the Zoom check box for a
magnified view of the histogram.
Dot Plot - The default location of the dot plot is the lower-right section of the Current
Batch Tab - The dot plot is a graphical display of real-time data collection. Luminex
recommends using the default settings to collect data.
Touring the Software
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