Luminex xPONENT 3.1 Rev 2 - Manual (Page 47)

Right-click inside the dot plot area to access the following options:
X Axis - Changes the X axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, and Classification 2
NOTE: You can change the X axis of the dot plot for troubleshooting
purposes. Use only the default settings in all other instances.
• Y Axis - Changes the Y axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, and Classification 2
• View - Choose to view all events or only gated events.
• Display Mode - Select either Logarithmic or Linear display. The software defaults to
Logarithmic display. Click maximize for a magnified view of the dot plot.
• Plot Type - Select either Density Plot or Decaying Plot. The software defaults to Density
Plot, which displays a constant accumulation of events. Contrasting colors indicate
increasing density. The Decaying Plot plots only the last 100 events.
NOTE: The Density Plot allows the visual elimination of data values that are
determined to be insignificant to the display. Luminex recommends
collecting data in Density Plot mode to observe all collected events.
You can only view the Decaying Plot during batch acquisition.
Right- click inside the dot plot area to access the following options:
Log - Displays a log of system processes. The log includes the following information:
• Date
• Message
• Code
Log entries indicating warnings are highlighted yellow. Errors are highlighted in red. Other log
entries are not highlighted. Click the maximize button to maximize the log. The log expands
to fill the whole window. Click minimize to return to the standard size.
Save Image - Opens a Save As dialog box to save a screen capture.
Details - Opens the Results tab, enabling more analysis and results. For more information,
see the “Results Tab” section.
Progress - Click to display real-time progress of the well acquisition. Analyte counts are
displayed in a dynamic bar graph as they are acquired. The scroll bar at the bottom of the
Progress display scrolls through the analyte list.
• Maximize - Maximizes the Progress display to fill the window.
• Zoom - Enable a closer look at the analyte progress.
• Save Image - Opens a Save As dialog box to save a screen capture.
• Default - Returns to the histogram and dot plot display.
Well Report - Displays a representation of the plate and the status of acquired wells. Wells
will display one of three possible states:
• Yellow - Well acquired, but the system detects a possible problem (select the Log tab for
more information).
• Green - Well acquired successfully.
• Red - Well acquisition unsuccessful, the system may have stopped, depending on the
circumstances (select Log tab for more information).
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