Techne PrimeQ - Manual (Page 145)

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3.16.2.1 Baseline correction
The choice of baseline correction method is similar to that shown in the Baseline Correction
Wizard (section 4.5) with the exception that Proportional is excluded. Click
Next when the baseline
correction is set.
3.16.2.2 Plus-minus analysis method
This screen allows the user to choose which readings are to be used for results scoring.
• End-point (default): Uses the last reading only (we recommend that >1 reading is used for
accuracy).
• All readings: Averages all readings in the stage
• Last readings: Averages a user-specified number of last readings (the default is 5, or 1 if
there are less than 5 readings)
• Specify range: Specify a range of readings to be averaged that best suit the data (the
default is the last reading).
3.16.2.3 Threshold settings
Clicking Next leads through to the Threshold Settings screen. In plus-minus scoring, a no
template control (NTC) is used to set confidence thresholds above which all unknowns are scored
as positive and below which as negative. Samples between the two threshold values are scored
as undetermined. This screen provides options for these threshold settings.
• Set the threshold range using the scroll-down menu.
The default settings are:
Upper threshold: Average of NTC samples + n SD (default 6 SD)
Lower threshold: Average of NTC samples + n SD (default 3 SD)