Techne PrimeQ - Manual (Page 81)

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• Analysis Method: The drop-down menu allows an analysis method to be selected.
• Dye name: The name of the dyes selected in the program setup will be displayed.
• Dye Usage: Assign a Dye Usage from the list in the drop-down menu. There will be one dye
usage box for each read present in the selection stage.
• Cancel: Aborts the procedure and takes the user back to the Experiment Editor.
• Finish: Accepts all the default analysis settings for the analysis method chosen and closes
the Wizard.
• Reset defaults: Pressing this button returns all analysis parameter settings to the default.
• Back/Next: Allows the user to move between screens in the Analysis Wizard.
The Analysis Method drop-down menu lists analysis types appropriate to the selected stage.
These will be dependent on the number of reads and the number of cycles programmed into the
run (see Choosing an analysis method in section 4.2)
PrimeQ supports the following analysis methods:
• No analysis method defined (None)
• Baseline correction
• Quantification, including:
a. Absolute quantification
b. Relative quantification
c. Relative quantification cycles (Cq)
• Dissociation curve