Techne PrimeQ - Manual (Page 15)

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1.1 PrimeQ
PrimeQ - the real-time nucleic acid detection system from Techne - has been designed with the
advantage of an open chemistry format that allows the end user full flexibility in the methods and
research they wish to pursue. This is complimented by the user-friendly application software,
Quansoft, for easy setup of experiments and rapid analysis of data.
1.1.1 PrimeQ features
• The white light source and PMT detector provide an impressive excitation range of 470nm
to 650nm and a detection range of 500nm to 710nm.
• Multiplex - multiple wavelengths are detectable per sample using up to four paired excitation
and emission filters housed in individual cartridge systems.
• Wide dynamic range up to at least nine orders of magnitude from starting copy number and
high sensitivity detecting down to 1.0nM fluorescein and single copy templates, depending
upon the assay.
1.1.2 The thermal system
• 96-well low-profile microplate format sealed with optical film.
• Temperature-controlled optical heated lid can be set between 100°C and 115°C (or off).
Designed to minimize loss of sample and prevent sample condensation.
• Ramp rates up to 2.2°C/sec.
• Temperature range 4°C to 98°C.
• Block uniformity of less than ±0.25°C.
1.2 Quansoft
Accompanying PrimeQ is Techne’s unique, intuitive wizard-based software, Quansoft. By
employing a series of user-friendly windows accessible from the home page, Quansoft enables
any real-time experiment to be created with ease.
1.2.1 Experiment Editor
By using combinations of plate layouts, thermal cycling programs and analysis parameters the
Quansoft Experiment Editor allows the user easy management of experimental protocols.
1.2.2 Plate Layout Editor
Within a matter of seconds a 96-well microplate can be assigned with blanks, controls, standards,
user-defined samples or unknown samples, all of which are colour-coded for ease of identification.