1 introducing quansoft – Techne PrimeQ User Manual

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3.1 Introducing Quansoft

PrimeQ is a real-time-PCR unit containing sophisticated thermal cycling and fluorescence
detection technology. The user interacts with the system via Quansoft, the user-friendly Windows-
based application software installed on a PC connected to the unit. The intuitive software allows
the user to perform three main functions:

1. Create a PCR program and define fluorescent reading points.

2. Run the program.

3. Detect and analyse the fluorescence readings.

At the heart of Quansoft lie the Editors; powerful software features that have been specially
designed to simplify setup, editing and customization of the experimental parameters. Plate layout,
thermal cycling program and analysis method parameters can all be conveniently accessed from
the Home page, with each Editor providing a flexible format and clear layout that makes viewing or
changing any aspect of the protocol simple and straightforward.

Quansoft Home page

By employing a series of user-friendly
windows, Quansoft enables any real-time PCR
experiment to be created with ease.

Experiment Editor

This powerful Quansoft feature acts much like
a shopping cart gathering together different
elements

of

an

experiment.

Using

combinations of plate layout and program
parameters, it offers the user a flexible
approach to the management of experiment
protocols.

Program Editor

Individual cycling steps/stages or ramps are
quickly added to build and display the thermal
program. Fluorescent reads can be added at
any step of a stage.

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