Techne PrimeQ - Manual (Page 153)

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3.17.3.1 Allelic discrimination scatter graph
Particular to the allelic discrimination method is the display of a scatter plot representing the
fluorescence of one allele plotted against the other. The software plots the results of the run on a
scatter plot of allele 1 vs. allele 2 (axes defined by the user in Reporter Selection) with each well of
the 96-well plate represented by a point on the graph.
The scatter graph shows the fluorescence values of each reporter in a given well plotted against
each other
• If the scatter graph is not displayed, click on the icon to bring up the data:
• To swap the axes over, return to the Reporter Selection screen in the Allelic Discrimination
Wizard.
Four distinct clusters can be seen representing the no template control (NTC) and three possible
genotypes: homozygous A (AA), or B (BB) and heterozygous (AB).
Homozygotes will show increased fluorescence along one axis of the plot (depending on which
reporter they contain), while the heterozygotes appear in the centre of the plot as they contain