Techne PrimeQ - Manual (Page 35)

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A message will appear informing the user to Please wait – checking cartridge status.
If the removal was performed correctly, the Cartridge Access and Editing table will be updated to
display “Not Fitted” next to the slot from which the cartridge was removed.
The table below gives details of the PrimeQ filter cartridges.
Filter
cartridge
Excitation
wavelength (nm)
Emission
wavelength (nm)
Suitable dyes
*FC01 460 500 FAM™ multiplex, Green
intercalating dyes, Fluorescein,
EvaGreen
®
FC02 485 520 FAM™, Green intercalating,
Fluorescein, EvaGreen
®
FC03 530 560 HEX™, TET™, JOE™, VIC ® ,
Yakima Yellow
®
FC04 580 615
ROX™, TEXAS RED ® , Cy ® 3.5
FC05 640 685
Cy ® 5, Quasar ® 670
*FC01 may be ordered separately.
1.16.8 Points to remember
• Filter descriptions are stored in the instrument memory and do not need to be re-entered if
the connected instrument is changed to a different PC. Filter descriptions are uploaded from
the instrument when Quansoft is opened.
• Changing the filter cartridge between experiments can only be carried out via the software –
it cannot be performed manually.
• It is critically important that the correct excitation and emission filters are in the assigned
position of the carousel.
• During multiplexing you must choose two fluorophores with different excitation and emission
wavelengths and measure the fluorescence with different filter cartridges.
• Avoid combinations of fluorophores with overlapping spectra.
• It is possible for some fluorophores to appear more than once on the dye list because more
than one filter pair could be used for its measurement. In this case, select the correct filter
by its wavelength properties.
1.17 Changing the instrument name
All PrimeQ units are shipped with the name “PrimeQ” stored in the instrument’s software. This can
be changed to a more appropriate name as required.
• To change the instrument name, click on the Maintenance icon on the navigation bar. Then
click on the Instruments icon. The default supervisor password is techne – we suggest you
change this as soon as possible by clicking on the Security icon. Please ensure that you
keep a record of the password, as without it you will not be able to access these functions.