Techne PrimeQ - Manual (Page 16)

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1.2.3 Program Editor
Individual cycle steps, stages or temperature ramps can be added quickly and easily together with
other parameters to build and display the thermal cycling program.
1.2.4 PrimeQ reports
Comprehensive, completely user-customizable reports can be generated from any of the analysis
methods. All result types including graphs can be exported to Microsoft
® Word, PowerPoint or
other programs currently used for publication purposes.
1.2.5 Data Analysis
Choose the analysis types and parameters from:
• None
• Baseline
• Quantification
• Dissociation curve
• Plus/minus scoring
• Allelic discrimination
• Multi-read (end point)
1.2.5.1 Quantification
A linearity of at least nine orders of magnitude can be achieved, allowing the user to quantify DNA
down to single copy template or to achieve absolute quantification of 1.0nM fluorescein in a
volume of 20µl.
1.2.5.2 Dissociation analysis
Using the easy-to-program ‘ramp’ function, PrimeQ will perform a dissociation curve that can be
used to determine the temperature at which the DNA strands dissociate. It provides the user with
extra confidence in genotyping experiments and in product verification analysis.
1.2.5.3 Plus/minus scoring
This analysis exploits PrimeQ’s fluorescence technology to determine with ease and accuracy the
presence or absence of a PCR product in any given sample.
1.2.5.4 Allelic discrimination
Users of PrimeQ have the option of this powerful technique capable of detecting single nucleotide
differences (SNPs). It can be used to discriminate between genotypes, mutations and
polymorphisms within or between samples simply by comparing the fluorescence signal obtained
using allele-specific, dye-labelled probes.