Techne PrimeQ - Manual (Page 94)

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A. User and experiment ID: A user name can be entered and comments specific to the
experiment can be added in the Comments box.
B. Results tabs: To the left of the main screen tab are a series of Results Editor tabs. The
uppermost (active) tab displays the Result Editor page as shown. The tabs then list the stages
in the order that they ran. Clicking on the tabs will display the results and graphs relevant to
that particular stage. It is also possible to move between stages from the Results Editor menu
bar.
• From the Analysis option, click on Show Stage and then the name of the stage to be
displayed.
• Also found on the results tabs is the Log/Audit Trail tab, which provides details of the run
useful for GLP purposes (see section 3.7.5), while the Report tab displays the information
sent to the PrimeQ report (see section 3.7.6).
C. Combine replicates: Clicking this button combines replicate readings to provide an average
for a sample. The button will then change to Show all Samples – click to revert to the
expanded data.
D. Dye to View: Where multiple readings have been taken, the reporter being displayed in the
graphs and results table can be changed using the drop-down menu. This option is disabled if
only one reporter is being used. Note that a PRD is not regarded as a reporter so if one of the
dyes was selected as a PRD in the Analysis Wizard, it will not be listed as available for
viewing.
E. Plate layout: The plate layout is always displayed and shows the colour-coded well types
(standards, unknowns etc.) as defined in the Plate Layout Editor.
• Fluorescent readings appear on a per-well basis with a small graph displayed inside each
sample well.
• Wells can be selected as in Microsoft
® Excel (click, ctrl-click, shift-click, drag, select
column/row/all), with only the selected wells appearing on the relevant analysis graphs.
• Hover the mouse over a particular well for information about the sample type given in a tool-
tip.
• Selected wells are persistent between dyes.
• Click outside the well plate on the top left hand corner for all filled wells to be viewed;
clicking again leaves all wells viewed but not selected (highlighted).
• Right mouse brings up a menu containing Flag wells (or ‘Unflag’ if flagged already). A
flagged well has a cross displayed and is removed from all calculations (appears in the