Techne PrimeQ - Manual (Page 37)

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1.18 LED power settings
The LED light source has variable power settings. This prevents PMT blinding and brings
fluorescence into its linear range for certain applications. The role of this option is to cut down
excess light from the LED source in high fluorescence applications. The three power options are:
Low (50mA), Medium (100mA), and High (200mA).
The power level is specified by the user when programming the PCR in the application software
(at the same time as specifying the fluorophore filter); the default setting is
Medium. The three set
power levels are not user-adjustable.
1.19 Inserting a plate
• To insert a plate, push the orange panel to release the latch, and open the drawer by sliding
it towards you.
• To close the drawer push it home until the latch clicks and the drawer stays shut.
1.19.1 Inserting a previously run plate
Users should note that when the thermal cycler plates are heated, the plastic may tend to distort
slightly. This means that if the plate is removed from the block then either returned or transferred
to another block, the fit can be poor. If returning or transferring a plate is unavoidable then the
following procedure is recommended to improve the fit:
• If a heat seal has been used, place the plate in the heat-sealer and seal the plate for 3-4
seconds; rotate the plate 180 degrees; seal the plate for 3-4 seconds.
• Insert the plate in the block and close the drawer.
1.19.2 Running a plate
Perform the loading procedure on the prepared plate following the instruction given when the
experimental run is started in the Quansoft operating software.