Techne PrimeQ - Manual (Page 154)

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copies of both alleles and therefore produce fluorescence with both dyes. Samples that do not
cluster tightly may contain rare sequence variations, sequence duplications or the PCR may not
have been optimal.
Assigning Genotypes
The user can define clusters of points within the plot that represent genotypic segregation within
the samples; the scoring of each sample is then presented in the Results table.
• Click Type Colour in the Display box.
• Select one of the coloured Type tiles in the Type box.
• To assign a sample to that type, either click on individual sample points or use the mouse to
draw a box around the cluster. To enhance visibility, maximize the scatter graph by clicking
on the zoom icon in the top-right corner of the pane.
The selected data points will change colour to that of the selected Type tile.
• Repeat for the remaining data until all genotypes have been defined.
Each type can be given a name which will then appear in the results
table.
• Click on Change name in the Type box and an Allele Type box
will open.
• Type in the required names and click OK to accept.