Leica Biosystems VT1200/VT1200 S - Manual (Page 22)

20 Instructions for Use V 1.4 RevC – 09/2013
5. Working with the Instrument
5.2.2 Fine-adjusting the blade-to-specimen proximity and feeding to the section thickness
The rotary knob 2 for moving the specimen receptacle vertically can be
used both for fine-adjusting the blade-to-specimen proximity and for adjust-
ing the desired section thicknesses. The step size: 1, 10 or 100 µm can be
selected by pressing the STEP SIZE key.
Turning the setting dial clockwise carries out the desired feed; turning the
setting dial counterclockwise lowers the specimen (minus sign). The value
appears in the lower display when "µm" is selected. After each sectioning
process has been completed, the µm display is set to zero.
Save a commonly used section thickness by adjusting the setting dial for
the section thickness and pressing the MEMO key for 3 seconds. An audible
signal confirms that the value has been accepted.
Feed to the saved value by quickly pressing the MEMO key. Pressing the
MEMO multiple times feeds to the value multiple times.
Blade
forward back
Neither negative values nor a section thickness of over 1000 µm are
permitted. If an attempt is made to save impermissible values, an au-
dible warning signal sounds three times and the last (permissible)
value is retained.
5.2.3 Moving the blade
The "Blade forward" and "Blade back" keys have to be held down until the
desired position is reached. The blade feed rate is 2.5 mm/s. When each end
point has been reached, the corresponding LED lights up in the key.