Leica Biosystems CM1510-S - Manual (Page 26)

26 Instruction manual V 1.4 – 04/2010
6.1 Specimen freezing
6.1.1 Quick-freeze shelf
The cryochamber is equipped with a quick-freezing shelf (Fig. 20) for up
to 10 specimen discs.
When working inside the cryochamber, keep hands away from
gilled evaporator – high risk of injury from sharp edges!
Specimen freezing
• Cut the specimen to size.
The specimen block should not be any bigger than 2 x 2 cm.
• Use a specimen disc at room temperature.
• Apply a sufficient quantity of cryocompound to the disc.
• Place the specimen on the disc and orient.
• Remove the cover from the quick-freeze shelf.
• Place the specimen disc with the specimen in one of the bores of the
quick-freeze shelf and freeze the specimen.
Specimen freezing on the quick-freeze shelf can be accelerated
by additionally using a mobile or a stationary heat extractor.
• Once the specimen is frozen, insert the specimen disc in the speci-
men head and start sectioning.
6.2 Inserting the specimen discs
Specimen discs are available in three sizes (ø 20, 25, 30 mm). The
grooved surface ensures firm contact with the specimen.
• A black dot (1) helps orienting the specimen.
Fig. 20
Fig. 21
6. Daily work with the instrument
1